Part:BBa_K3838234:Design

YH-BSH

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 9
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 9
  Illegal NotI site found at 15
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 9
  Illegal BglII site found at 1046
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 9
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 9
  Illegal XbaI site found at 24
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The nissle1917 will have a low level of expression of BSH gene.

Source

BSH gene is from Lactobacillus plantarum strain MBUL90.